A simple RT-PCR-based strategy for screening connexin identity
نویسندگان
چکیده
منابع مشابه
A simple RT-PCR-based strategy for screening connexin identity.
Vertebrate gap junctions are aggregates of transmembrane channels which are composed of connexin (Cx) proteins encoded by at least fourteen distinct genes in mammals. Since the same Cx type can be expressed in different tissues and more than one Cx type can be expressed by the same cell, the thorough identification of which connexin is in which cell type and how connexin expression changes afte...
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The technology of laser-capture microdissection (LCM) enables the easy extraction of single cells or defined groups of cells from a tissue section (1,2). This technique uses a laser pulse to activate a plastic polymer, which binds to and captures cells from a frozen or paraffin-embedded tissue section. Captured cells present on this solid support can then be used for nucleic acid and/or protein...
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bromide staining in detecting PCR products (Figure 3). Moreover, as expected, band intensities increase with longer duration of exposure to X-ray film (data not shown). Biotin-21-dUTP and biotin-14-dATP work equally well in labeling the PCR products (data not shown). Similarly, DNA cross-linking with baking and UV irradiation work equally well in this system (data not shown). We have also confi...
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Discrimination between the amplification of mRNA and contaminating genomic DNA is a common problem when performing a reverse transcriptase-polymerase chain reaction (RT-PCR). Even after treatment of the samples with DNAse, it is possible that negative controls (samples in which no reverse transcriptase was added) will give positive results. This indicates that there was amplification of DNA, wh...
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 1999
ISSN: 0100-879X
DOI: 10.1590/s0100-879x1999000800014